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mTOR inhibitors like rapamycin offer promise by enhancing mitochondrial autophagy, yet their immunosuppressive propertiesincluding increased susceptibility to infections and potential neoplastic effectslimit long-term clinical use

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Purified RNA was quantified at OD 260 nm using a Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific, USA) and qualitated using a 2100 Bioanalyzer (Agilent Technologies, USA) with an RNA 6000 LabChip nano assay kit (Agilent Technologies, USA)

For example, oxidative damage to tryptophan hydroxylase, the rate-limiting enzyme in serotonin synthesis, can decrease serotonin levels in the brain (Correia and Vale 2024