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Cells were then washed three times with ice-cold PBS and incubated for 1 h at 4 C with 1 mM bis(sulfosuccinimidyl) suberate (BS3, non-cell permeable cross-linker, Thermo Scientific) in PBS

The injections take some getting used to (intramuscular) but Im serious about my health so I got used to it

They regulate many biological functions, including: Tissue repair Immune response Inflammation Hormone signaling Collagen production Injectable peptides are synthetic or modified versions of these naturally occurring molecules, formulated to target specific pathways that may promote healing

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