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Cystine can enter the lysosomes via cystine-containing proteins or enter the cell via apical membrane transporters

doi: 10.1152/physrev.2001.81.3.1065 25

After each chromatographic separation, fractions must be analyzed to determine the fractions that contain the protein of interest and the relative purity of each of those fractions

In the central nervous system, ammonia can cross the bloodbrain barrier and is normally detoxified by astrocytic glutamine synthetase, but whether brain metastatic tumor cells exploit or tolerate this ammonia-rich environment remains unclear ( From a clinical-translational perspective, elevated ammonia is not merely a metabolic byproduct but actively drives immune evasion and therapeutic resistance