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Immunoprecipitation Immunoprecipitation (IP) of NHR-49::GFP was performed using either GFP-Trap Magnetic agarose beads (Cat

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The treated RBCs were stained with EMA (20 L of RBC with concentration 0.5 10 9 cells/mL were added to 80 L of HEPES-buffer with 0.1 L of 5 g/ml EMA solution and incubated for 30 min)