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The (+) strand standard was quantified by spectroscopy and diluted to indicated concentrations

In previous years, ferroptosis has been shown to serve a key role in numerous pathological processes, including neurodegenerative diseases, cancer and inflammation-related disorders (3)

When abundantly incorporated into membrane phospholipids, PUFAs greatly increase cellular susceptibility to ferroptosis due to their high autoxidation potential ( 3.1.2 Expression and regulation of key enzymes Acyl-CoA synthetase long-chain family member 4 (ACSL4) and lysophosphatidylcholine acyltransferase 3 (LPCAT3) are key enzymes that modulate phospholipid synthesis and remodeling

For the cross-sectional study, all sampling occasions were combined prior to being fit to a linear mixed effects model, with age group as the fixed effects and individual cat as the random effects