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Spirometry and lung volumes are normal or minimally impaired (opposing effect between the hyperinflation due to the emphysema and the loss in volume due to the fibrosis), but there is a marked and disproportionate decrease in the DLco and significant exercise-induced hypoxemia (probably due to the additive effect of the emphysema, fibrosis and pulmonary vascular disease)

Immunofluorescence staining also showed a marked increase in the number of Ki-67-positive cells and a decrease in the nuclear content of -H2AX and P21 after FOXO4-DRI treatment (Figures 4B,C)

The half-life of peroxynitrite is short (~1020 ms), but sufficient to cross biological membranes, diffuse one to two cell diameters (312), and allow significant interactions with most critical biomolecules (1041)

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