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Cultures of Tn5 libraries were initiated with 100 l of thick cell scrape mixture in 100 ml LB supplemented with 50 g/ml kanamycin, Mg 2+ , and Ca 2+ , to an initial OD 600 value of 0.10.15

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These methodologies are critical innovations tailored to Southeast Asias challenges: eDNA enables non-invasive detection of cryptic or rare Odonata taxa in remote, fragmented habitats, facilitating large-scale biomonitoring where traditional surveys are challenging 20,21,22