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All cell lines were maintained in 10 cm tissue culture plates (Corning Falcon) and sub-cultured using 0.025% trypsin-EDTA (Gibco)

The brain is particularly susceptible to oxidative stress given its limited antioxidant enzymatic capacity to counteract reactive oxygen species production (Fisher-Wellman and Bloomer, 2009), habitual RT has been associated with a reduction in markers of oxidative stress at rest, with the bulk of evidence coming from animal models

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