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Moreover, the introduction of novel molecular componentssuch as fusion proteins or transplanted mitochondriaposes immunogenicity risks, as these elements may be recognized as foreign antigens and elicit host immune rejection

Rizzo A, Roscino MT, Binetti F, Sciorsci RL

From Day 0 to Day 2, the medium was replaced daily with N2 medium consisting of DMEM-F12/Glutamax (Thermo Fisher Scientific 10565042), N2 supplement (Thermo Fisher Scientific 17502048), BDNF (20 ng/ml, Peprotech 450-02), Lam (1 g/ml, Thermo Fisher Scientific 23017-015), MEM-NEAA (1X, Thermo Fisher Scientific 11140050), and doxycycline (4 M, STEMCELL Technologies 72742)