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The mitochondrial pellet was resuspended in MSHE buffer with BSA and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific)

Another mechanism of GSH protection is reverse post-translational modifications of proteins by S-glutathionylation/deglutathionylation), which is common for the Krebs cycle and the electron transport chain proteins catalyzed by glutathione-S-transferase and glutaredoxins (Mar et al., 2020)

Neurotransmitter Regulation: SAMe is involved in the production and regulation of various neurotransmitters in the brain and nervous system

Dosing protocols and administration Both mazdutide and retatrutide are administered as once-weekly subcutaneous injections