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2.10.1 Limitation of in-vitro model Despite their value in mechanistic research, in vitro models present several critical limitations that constrain their translational applicability (Slanzi et al., 2020)

Knockdown of Notch3 can increase the ROS level in lung cancer cells, reduce the expression levels of GPX4 and peroxiredoxin 6 (PRDX6), and then induce lipid peroxidation to trigger cell ferroptosis [178]

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