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Owing to significant differences in metabolite concentrations, the detection of low-concentration metabolites demands increased sensitivity from these technologies

In patients who had elevated level of serum lactate dehydrogenase (LDH) (key glycolysis rate-limiting enzyme), we found a moderate increase in the expression of hif-1 and lag-3 (Supplementary Fig

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Consequently, non-enzymatic crotonylation is regarded as a protein modification mechanism that directly and rapidly responds to changes in cellular metabolic state, working in concert with the canonical enzymatic modification pathway to constitute a dynamic and complex regulatory network for crotonylation [126]