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RNAi-expressing bacteria were allowed to grow overnight at 20 C

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Particles were split into two datasets and refined independently, and the resolution was determined using the 0.143 cutoff (Fig S2E)

In addition to assessing irreversible inhibitors, our GSH reactivity assay has been adapted and optimised to identify the presence of a reversible covalent inhibitors by establishing an equilibrium between the GSH adduct and the parent compound