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The patients with glaucoma also underwent measurement of mean deviation (MD) with the Humphrey field analyzer (HFA)

The calculation of % inhibition was performed using the following formula: Where: A sample = Absorbance of Nar-NP treated sample

Significantly, we show DA functions as a biased agonist at 2A -adrenergic receptors, preferentially signaling via the canonical G protein-mediated pathway

The inactivation of the pure enzyme depended on the time and temperature of incubation (t 1/2 = 2 min at 37C), and was proportional to the |INADPH|/|enzyme| ratio, reaching 50% in the presence of 0.3 M NADPH and 45 M NADH respectively, at a subunit concentration of 20 nM