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Immunoblotting Cell lysates were extracted in 1 RIPA lysis and extraction buffer (Sigma-Aldrich Chemie GmbH R0278) containing 2 Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific 78444) for 20 min on ice

Subsequently, ten patients with ROC were pretreated with low-dose cyclophosphamide to suppress regulatory T cells (Tregs) and enhance the clinical efficacy of p53-SLP vaccine