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NRN1 is uniquely positioned as it can target multiple pathophysiological drivers of glaucoma, including lack of neurotrophic support and aberrant intracellular signaling, ultimately addressing the critical endpoint of glaucomas pathophysiological process: the loss of RGCs

Samples were centrifuged at 4 C for 15 min at 14,000 g , and the supernatant was collected and diluted 1:1000 in LC graded H 2 O before untargeted metabolomics analysis

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