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2.18 Multiplex enzyme-linked immunosorbent assay profiling of TCM and NCM To assess angiogenic, vascular injury, pro-inflammatory, cytokine and chemokine secretions a 54-plex ELISA kit separated across 7 plates was used (Meso Scale Discovery, Rockville, MD, USA)

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The media was then aspirated from the SVKCR2 cells and replaced by the antibody-virus mixture

70 Further research indicates that aside from inherent antimelanoma activity, NBDHEX also has the ability to enhance the function of temozolomide, with the two able to work synergistically to suppress tumor growth