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This result is opposite to the report of O.oeni PSU-1 under wine like medium (1 h), and similar with the situation after 6 h inoculation (Margalef-Catal et al., 2016), meanwhile it indicate that when cells are exposing at low pH, the ATPase activity is increased more quickly than wine like medium, and agrees with the role of this enzyme in the regulation of the cytoplasmic pH and in the acid stress response of O

Characterization of grape musts and wine Free and total SO were determined by the Rippers method, as described by On the other hand, pH was determined using a pH meter HI2221 (Hanna instruments, United States)

Total protein (3040 g) was loaded in 10% and 420% acrylamide gel (Bio-Rad, Hercules, CA, USA) and transferred onto a polyvinylidene fluoride (PVDF) membrane and incubated with first antibodies in T-TBS with 1% bovine serum albumin for 12 h at 4 C

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