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UBE2D2 and Ub were isolated using cation-exchange chromatography and eluted with either 50 mM HEPES pH 7, 250 mM NaCl, 5 mM DTT or 25 mM sodium acetate pH 4.5, 250 mM NaCl, respectively

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Conversely, chronic NAD+ depletion during aging can impair both PARP-mediated DNA repair and sirtuin-mediated stress resistance, contributing to accelerated aging phenotypes

From the side of the kidneys and urinary tract: increase and / or decrease in the frequency of urination, impaired urination, dilatation of the urinary tract, impotence, testicular edema, gynecomastia, breast enlargement, galactorrhea