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In recent years high anion gap acidosis due to acquired 5- oxoprolinuria has been reported in association with chronic paracetamol use 1

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Weinberg SE, Sena LA, Chandel NS

First, we sought to identify a medium that would support assembloid formation, that is, the culture of all three cell types: hepatocytes, cholangiocytes/ductal cells and portal mesenchyme without overgrowth of any of them, we tested several media and found that a minor adaptation of the DM medium used for h-HepOrgs differentiation without A8301 (assembloid medium) supported culture of the three cell types while preventing their overgrowth