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Cells were transfected with either pcDNA3.1-GFP control (1 g DNA per 1.5 10 5 cells) or pcDNA3.1/hPPAR (1 g DNA per 1.5 105 cells, OriGene, NM_000030)

discovered that comutations in STK11 and KEAP1 suppress SCD1 expression, thereby promoting MUFA synthesis and reducing PUFA levels, which ultimately suppresses ferroptosis in lung cancer cells [78]

Thus, we hypothesized that there might be biological implications to the observed variability in speciofoline levels across kratom extracts (Table 1)

Immediately after electroporation, 80 l of warm growth medium was added to each well and the strip incubated at 37 C for 30 min