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Kyn, 3OHK and 3OHKG are found prominently in young lenses (102), but decline at a rate of ~12% per decade, with kyn and 3OHK being nearly undetectable in 80 year old lenses (110)

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To test the function of the cyclin D-Rb interaction, we examined immortalized human mammary epithelial cells (HMECs) expressing doxycycline-inducible Rb variant proteins fused to Clover fluorescent protein and 3FLAG affinity tags (Figure 5A-B and S6A-B)