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Mitochondrial isolation SNr mitochondria were isolated from the freshly dissected tissues of anesthetized with pentobarbital sodium (100 mg/kg, i.p.) mice using commercial mitochondrial extraction kits (Solarbio, SM0020, China)

PPAR-s ability to boost the transcription of genes exhibiting antioxidant activity, including glutathione peroxidase, catalase, SOD, and HO-1, accounts for its antioxidant action

There is a significant age-dependent increase in ferroptosis in the kidney, spleen, liver, ovary, uterus, cerebellum, and bone marrow, accompanied by iron accumulation 41

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