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Furthermore, JHU083 has been reported to enhance the proliferation of CD8 + T cells and improve the efficacy of PD-1 blockade in hepatocellular carcinoma [151]

Rotenone, and other complex I inhibitors, such as MPTP, paraquat and maneb, are used as models for assessing the environmental causes of PD [12]

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Cells were rinsed twice in PBS and then lysed in M-Per buffer (78503, ThermoFisher Scientific) containing Halt Phosphatase Inhibitor (78428, ThermoFisher Scientific) and Halt Protease Inhibitor (78437, ThermoFisher Scientific), both diluted 1:100