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The supernatant was discarded, and the total RNA was dissolved in 30 L of DEPC-treated water

But despite being one of the most commonly used laboratory reagents in peptide research, bacteriostatic water generates a steady stream of questions from Canadian researchers: what is it actually, does it need refrigeration, how long does it last, how does it differ from sterile water, can you use it for multiple peptides, and what happens if you use the wrong kind of water instead

We do not recommend B12 injections unless a true deficiency is present, in line with current medical guidelines

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