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To obtain bacteriological sterility prior to conducting immunopeptidomics experiments, the cell pellet was lysed using 2x lysis buffer and complete protease inhibitor cocktail (Roche) for 30 minutes on ice, with periodic shaking every 10 minutes

Depending on how you're finding target identification, you might also want to play around with the color options for enemy UI under the 'Color Blind' options - some stand out a lot more than the default red one