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Membranes were incubated overnight at 4 C with the following primary antibodies: phospho-ERK T202/Y204 (1:1,000), phospho-PKC (1:1,000), caspase-3 (1:1,000), caspase-7 (1:1,000), caspase-8 (1:1,000), caspase-9 (1:1,000) and GFP-tag (1:2,000)

Once sessions stop, the skin slowly returns to its original tone over time

After 1 h the cells were washed with OPTI-MEM and incubated with 20 M of dichlorofluorescin diacetate (DCFH-DA, Sigma) for 30 min at 37 C and 5% CO 2

The thyroid gland contains iodine, this element and Se have a synergistic relationship for a healthy thyroid gland and therefore both these elements in proper amounts are needed