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However, the optimal dosage, administration route and long-term safety of NADPH precursors remain unvalidated in large animal models, and the crosstalk of NADPH metabolism, iron homeostasis and retinal inflammation is poorly understood, limiting clinical translation of NADPH-targeted therapies and requiring further investigation

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The copper ion is not merely a passenger in this complex it is integral to the peptide's biological activity, as GHK without copper shows significantly diminished effects in many assays