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To validate that R4VPs-induced cell death is due to the loss of RNF4, we first mapped the binding site(s) of R4VPL3-1 to RNF4 using a biotin-tagged R4VPL3-1, in which the VHL-r moiety was replaced with biotin (Biotin-R4VPL3-1)

Photoactivation of H1.4-PAGFP was initiated at 405 nm within an 8 m diameter region (35 pixels)

Bioscientia Medicina

For example, the leucine-rich repeat kinase 2 ( LRRK2 ) G2019S mutation delays mitochondrial arrest and promotes PD onset by preventing MIRO1 removal from the OMM, thereby reducing OPTN-dependent mitophagy