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Generation of sh EPAS1 cell lines Lentiviral vector plasmids, packaging plasmids, and transfection reagent Lipofectamine 3000 (Invitrogen, USA) were mixed and added to 293T cells to generate lentiviral particles

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With the ongoing accumulation of intracellular iron ions, a notable increase in the levels of iron cycling phase proteins, including DMT1 and TFR1, occur within both osteoblasts and osteoclasts (131, 132)