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But deep in his rebellious soul, he felt that Burnet had stripped the natural selection theory of one basic feature, namely, that the antigen encounters first the circulating antibody, rather than receptors of the antigen specific cells

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Primary mouse peritoneal macrophages, mouse macrophage-like RAW 264.7 cells, and human monocytic THP-1 cells were transfected with GSTP encoding expression vector (pcDNA3.1-Flag-GSTP) or empty vector (pcDNA3.1), followed by stimulation of LPS (500 ng/ml) for 18 h, and the levels of HMGB1 in the culture medium were determined by ELISA

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