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Sequencing was performed using a HiSeq 2500 (Illumina), with a read length of 300 bp paired-end reads and at least 25,000 tags per sample

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Terminal labeling contralateral to injury: polyethylene glycol-fused animals Rostrocaudal extent At all post-operative timepoints following either Single Cut or Allograft PEG-fusion repair, animals reliably exhibited terminal labeling contralaterally (29 out of 29 cases) within the correct boundaries around the L3/L4 segmental division established in Unoperated Control animals ( Figure 7A )