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Location Gallstones can grow in different tracts of the biliary tree and their localization governs the choice of the appropriate therapeutic approach

Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPRCas gene editing

The PCR reaction was carried out in a final volume of 25 l containing 2X PCR master mix (EmeraldAmp GT PCR Master Mix, Takara Bio Inc., Shiga, Japan), 20 pM of each primer (forward primer: 5'TGAAGGAGAAGGTGTCTGCGGGA3' and reverse primer: 5'AGGACGGTGCGGTGAGAGTG3' synthesized from Sigma-Aldrich Chemical Pvt Limited, (Milwaukee, Wis., USA), 100 ng of the DNA template and nuclease-free water

[DOI] [PubMed] [Google Scholar] 46.Simic P., Pljesa I., Nejkovic L., et al