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It is not the most hands-on intensive protocol, so usually you can read a paper or design a new plasmid as you clean your beads in the background

We focus on hydration and recovery support delivered comfortably at your location

Furthermore, primer design for RT-qPCR experiments (Table S3) was executed using Primer Premier software (version 5.0), and primer specificity was confirmed using the Primer Check function of TBtools-II software