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Description

We downloaded legacy RNA-seq counts data from the Genomic Data Commons (GDC) portal using TCGA biolinks [64] and performed log2-cpm normalization using edgeR, after correcting for library size using the TMM method [65]

Specifically, eligible studies included participants (P) with a confirmed diagnosis of vitamin B12 deficiency, interventions (I) involving oral or sublingual vitamin B12 administration, comparators (C) using intramuscular supplementation, and outcomes (O) reporting changes in serum vitamin B12 or homocysteine levels