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Nat Rev Mol Cell Biol 9:112124

Today, we refine this

Ahuja, P., Ng, C

The lysate was filtered with a 0.45 m filter and loaded onto a Ni-NTA (Carl Roth, Germany) gravity column equilibrated with buffer A (20 mM sodium phosphate pH 7.5, 500 mM NaCl and 12.5 mM imidazole)