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Ethics declarations Ethics approval The procedures and protocols used in this study were designed in accordance with the guidelines for animal welfare and the use of animals prepared by the Institutional Animal Care and Ethical Committee of Taif University, Saudi Arabia, for project TURSP-202009

The total number of the cells was evaluated based on the following formula: where Q was the total number of sexual lineage cells observed in all disectors, h was the optical disector's height, a/f was the area of the counting frame, P was the total number of observed frames, BA was the setting of the microtome, and t was the mean of the final section thickness [36]

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