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Plots marked with three regions of ligandability changes: ligandability changes align with abundance changes (region 1), ligandability changes occur without corresponding abundance changes (region 2), and ligandability changes occur in the opposite direction of abundance changes (region 3)

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Recently, modern alternatives to such distribution and access challenges have come to light, such as single-dose slow-release implants, film-based vaccines 94 , and microneedle-based patches that could reduce reliance on the cold chain and ensure vaccination even in situations where qualified HCP are rare or in high demand

However, many factors, including their low stability, short half-life, high immunogenicity, tissue targeting, cellular uptake, and endosomal escape, hamper the delivery and clinical application of NADs