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After vortexing for 1 min at 48 C and incubated for 4 h at 80 C, the samples were then centrifuged at 14,000 g for 10 min at 4 C

The form of glutathione significantly impacts whether the molecule survives the gastrointestinal environment, how it is absorbed, and whether it successfully raises intracellular GSH levels in target tissues

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The elucidation of the molecular structure of the unknown Hg peak by ESI-MS revealed a GS-HgEt adduct, which allowed to propose a degradation mechanism for THI at pH 7.4 (Degorge et al., 2025), which may unfold in RBCs (Figure 2)