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They consist of two main components: the nucleus pulposus (a gel-like center) and the annulus fibrosus (the tough outer layer)

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2.3 Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)