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doi: 10.3390/ijms25021344 67 AitkenRJ

This method uses slightly longer needles, usually 22 to 25 gauge and 1 to 1.5 inches, depending on muscle mass and body composition

Hepatocytes were transfected with 2.75 M dsiRNA (Integrated DNA Technologies, Coralville, IA) in nuclease-free duplex buffer using the Viromer Blue transfection kit (Origene) following the manufacturers protocol