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72 A 2021 meta-analysis of 16 RCTs, involving 987 patients with SUDs, found a significant decrease in craving symptoms and a significant improvement in depressive and withdrawal symptoms with NAC compared with control, whilst lower smoking frequency failed to reach statistical significance

Science 274 , 252255 (1996)

After blocking, the blocking buffer was removed and the AIM solution with various concentration of CaCl 2 was added to the well and incubated for 1 h at room temperature