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Pineal Res.,26147152 69

Testosterone was used to simulate the PCOS cell culture environment

Fujita N, Sugimoto R, Takeo M, Urawa N, Mifuji R, Tanaka H, et al

Quantitative real-time PCR (Q-PCR) Total RNA was extracted from control and treated cells using the RNeasy Mini Kit (Qiagen) and following the protocol provided by the manufacturer