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Excessive ROS can also damage the mitochondrial respiratory chain, resulting in insufficient ATP production, reduced membrane potential, altered membrane permeability, and calcium overload, all of which contribute to mitochondrial dysfunction

Table1 Binding to thiol/sulfhydyryl groups of these proteins can firstly result in structural modifications of these proteins, which in turn negatively effects the catalytic capacity and thereby reducing or blocking the metabolic capacity to neutralize reactive oxygen species (Fernandes, 1996

What differs between these assays is the sensors solubility

doi: 10.1016/S1734-1140(13)71521-4 55 MishraRKAlokamRSriramDYogeeswariP