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Immunoblotting Cells were lysed in protein lysis buffer (Cell Signaling) supplemented with Roche complete Protease Inhibitor Cocktail tablets (Sigma-aldrich)

However, according to a new study, pure fractions of flavonoids from okra plant flowers had a strong anticancer effect on colorectal cancer in both in vivo and in vitro settings, exhibiting substantial anti-proliferative effects on tumor growth and strong antioxidant properties [13]