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After the collection of urine the animals were placed in their respective cages, and provided with food and water

Fernandez-Guerra P, Gonzalez-Ebsen AC, Boonen SE, Courraud J, Gregersen N, Mehlsen J, et al

Whether the consequences of cuprizone are directly related to copper chelation is unclear given that copper supplementation has limited capacity to reduce cuprizone toxicity to the CNS of mice [24]