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After being stirred continuously at 60 C for 2 h, the mixture was cooled to room temperature naturally

TLRs are pattern recognition receptors expressed by immune cells and epithelial cells that assist the host in differentiating between pathogenic and commensal microorganisms by recognizing pathogen-associated molecular patterns (PAMPs), conserved structures specific to pathogenic and non-pathogenic microorganisms, or damage-associated molecular patterns (DAMPs) ( TLR2 is widely activated in GERD patients, and activated TLR2 regulates the epithelial barrier, epithelial cell proliferation, and inflammatory responses of the body, which in turn are involved in the development of GERD

Moreover, involving civil society and Istanbul residents in decision-making and implementation processes concerning urban services will contribute to making these services more inclusive, spatially just, and accessible, thereby reinforcing the citys resilience goals