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Description

The standard procedure and reagents from the Q5 Site-Directed Mutagenesis kit (NEB) were used to insert mutations, ligate plasmids and transform them into NEB 5 competent cells by heat shock

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SH-SY5Y and BV-2 cells were grown in 6-well plates at densities of 1 106 and 5 105 cells/well, respectively

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Postdoctoral Fellow, Moore Laboratory Kayla Sipple Research Technician, Department of Neurodegenerative Science Erin Williams, Ph.D

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