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Gln inhibits miR-29b-3p transcription via c-Myc regulation to promote GGCT protein expression To determine GGCT downregulation during Gln deprivation, we first excluded protein degradation pathways as proteasomes (MG132) or lysosomes (CQ) inhibition failed to restore GGCT levels (Fig

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Linearized template was transcribed using the MEGAscript T7 Transcription Kit (Invitrogen) followed by capping with New England Biolabs Vaccinia Capping System as previously described 73

Most evidence is still early-stage or preclinical