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To obtain MSC, culture cells were grown in DMEM-F12, supplemented with 10% Fetal Bovine Serum (FBS) (Thermo Fisher Scientific, USA), 1% ITS (Corning, USA), and 100 g/ml primocin (InvivoGen, USA) until confluent, then propagated and passaged 26 times

Secondary outcomes included post-LT disease recurrence defined as alcohol relapse based on interview or alcohol biomarkers as per NIAAA definitions and/or steatosis on imaging, including transient elastography

It reflects a broader shift: patients are researching peptide therapy as a serious alternative to lifestyle-only approaches to stubborn visceral fat and metabolic decline

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